Anticorps Monoclonal anti-APOH

APOH Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

4D9A4

N° de cat : 66074-1-PBS

Synonymes

Apolipoprotein H, 4D9A4, Activated protein C-binding protein, Apo H, Apo-H

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Galerie de données de validation

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  WB analysis using 66074-1-Ig (same clone as 66074-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66074-1-Ig (same clone as 66074-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66074-1-Ig (same clone as 66074-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  WB analysis of pig plasma using 66074-1-Ig (same clone as 66074-1-PBS)

  pig plasma were subjected to SDS PAGE followed by western blot with 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66074-1-Ig (Apolipoprotein H antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66074-1-Ig(Apolipoprotein H antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66074-1-Ig (same clone as 66074-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (66074-1-Ig, Clone: 4D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66074-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66074-1-PBS cible APOH dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOH Protéine recombinante Ag17915
• Nom complet: apolipoprotein H (beta-2-glycoprotein I)
• Masse moléculaire calculée: 345 aa, 38 kDa
• Poids moléculaire observé: 50-53 kDa
• Numéro d’acquisition GenBank: BC026283
• Symbole du gène: APOH
• Identification du gène (NCBI): 350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins.

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