Anticorps Monoclonal anti-Arfaptin-1

Arfaptin-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A10B3

N° de cat : 67461-1-PBS

Synonymes

ARFIP1, Arfaptin 1, ADP-ribosylation factor-interacting protein 1, 2A10B3

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Galerie de données de validation

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  WB analysis using 67461-1-Ig (same clone as 67461-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67461-1-Ig (same clone as 67461-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67461-1-Ig (same clone as 67461-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67461-1-Ig (same clone as 67461-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67461-1-Ig (same clone as 67461-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Arfaptin-1 antibody (67461-1-Ig, Clone: 2A10B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67461-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51228-2

  Cytometric bead array standard curve of MP51228-2, Arfaptin-1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67461-3-PBS. Detection antibody: 67461-1-PBS. Standard:Ag12097. Range: 3.125-100 ng/mL.

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Informations sur le produit

67461-1-PBS cible Arfaptin-1 dans les applications de WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arfaptin-1 Protéine recombinante Ag12097
• Nom complet: ADP-ribosylation factor interacting protein 1
• Masse moléculaire calculée: 373 aa, 42 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC103759
• Symbole du gène: Arfaptin-1
• Identification du gène (NCBI): 27236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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