Anticorps Recombinant de lapin anti-Arginase-1

Arginase-1 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4M7

N° de cat : 82975-1-PBS

Synonymes

ARG1, Arginase 1, liver Arginase, 4M7, ARG 1

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Galerie de données de validation

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  WB analysis using 82975-1-RR (same clone as 82975-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82975-1-RR (Arginase-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82975-1-RR (Arginase-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 82975-1-RR (same clone as 82975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 82975-1-RR (same clone as 82975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82975-1-RR (same clone as 82975-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Arginase-1 antibody (82975-1-RR, Clone: 4M7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82975-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82975-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82975-1-RR against Human Arginase-1 were performed. The affinity constant is 4.77 nM.

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Informations sur le produit

82975-1-PBS cible Arginase-1 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Arginase-1 Protéine recombinante Ag8595
• Nom complet: arginase, liver
• Masse moléculaire calculée: 236aa,25 kDa; 322aa,35 kDa
• Poids moléculaire observé: 35-36 kDa
• Numéro d’acquisition GenBank: BC005321
• Symbole du gène: Arginase-1
• Identification du gène (NCBI): 383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

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