Anticorps Monoclonal anti-Arginase-1

• Phare
• Validé par KD/KO

Arginase-1 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

IF-P

Conjugaison

CoraLite®532 Fluorescent Dye

CloneNo.

5D6D12

N° de cat : CL532-66129

Synonymes

ARG1, Arginase 1, liver Arginase, 5D6D12, ARG 1

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Galerie de données de validation

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  IF Staining of rat liver using CL532-66129

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite®532 Arginase-1 antibody (CL532-66129, Clone: 5D6D12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu hépatique de rat,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL532-66129 cible Arginase-1 dans les applications de IF-P et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arginase-1 Protéine recombinante Ag8810
• Nom complet: arginase, liver
• Masse moléculaire calculée: 236aa,25 kDa; 322aa,35 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC005321
• Symbole du gène: Arginase-1
• Identification du gène (NCBI): 383
• Conjugaison: CoraLite®532 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 537 nm / 560 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

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