Anticorps Recombinant de lapin anti-Arginase-2

Arginase-2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242362A9

N° de cat : 84808-5-PBS

Synonymes

ARG2, 242362A9, ARG 2, Arginase 2, Arginase II

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Galerie de données de validation

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  WB analysis using 84808-5-RR (same clone as 84808-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control. This data was developed using the same antibody clone with 84808-5-PBS in a different storage buffer formulation.

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  WB analysis using 84808-5-RR (same clone as 84808-5-PBS)

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84808-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84808-5-RR (same clone as 84808-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84808-5-RR (Arginase-2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84808-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84808-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84808-5-RR against Human Arginase-2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84808-5-PBS cible Arginase-2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Arginase-2 Protéine recombinante Ag6609
• Nom complet: arginase, type II
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC001350
• Symbole du gène: ARG2
• Identification du gène (NCBI): 384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

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