Anticorps Recombinant de lapin anti-Arginase-2

Arginase-2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242362A9

N° de cat : 84808-5-RR

Synonymes

ARG2, 242362A9, ARG 2, Arginase 2, Arginase II

---

Galerie de données de validation

• 

  WB analysis using 84808-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. Mouse liver tissue as a negative control.

• 

  WB analysis using 84808-5-RR

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 84808-5-RR (Arginase-2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 84808-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84808-5-RR (Arginase-2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84808-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84808-5-RR against Human Arginase-2 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules U-251, cellules Caco-2, cellules K-562, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84808-5-RR cible Arginase-2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Arginase-2 Protéine recombinante Ag6609
• Nom complet: arginase, type II
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC001350
• Symbole du gène: ARG2
• Identification du gène (NCBI): 384
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase 2 is composed of 354 amino acid residues, including an NH2-terminal presequence for mitochondrial targeting and import. In the mitochondria, ornithine generated by Arginase 2 will give rise to glutamate via ornithine aminotransferase (OAT). Glutamate participates in several transamination reactions, including forming α-ketoglutarate (αKG) that may enter the TCA cycle and increase cycle intermediates and flux.Arginase 1 is mainly expressed in hepatocytes, and mice with a disruption of Arginase 1 gene die soon after birth. Arginase 2 is poorly expressed in hepatocytes, and most highly expressed in kidney, prostate, and immune cells such as monocyte/ macrophages. (PMID: 25234945,PMID: 27214549)

{{ptg:RelatedPrimaryAntibodies}}