Anticorps Polyclonal de lapin anti-Arp5/ACTR5

• Phare
• Validé par KD/KO

Arp5/ACTR5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21505-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 21505-1-AP (same clone as 21505-1-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  WB result of Arp5/ACTR5 antibody (21505-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  WB analysis using 21505-1-AP (same clone as 21505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 21505-1-AP (same clone as 21505-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 21505-1-AP (same clone as 21505-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 21505-1-AP (same clone as 21505-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 21505-1-AP (same clone as 21505-1-PBS)

  IP result of anti-Arp5/ACTR5 (IP:21505-1-AP, 4ug; Detection:21505-1-AP 1:500) with HEK-293 cells lysate 1120ug. This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 21505-1-AP (same clone as 21505-1-PBS)

  Immunofluorescent analysis of A549 cells using 21505-1-AP (Arp5/ACTR5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21505-1-AP (same clone as 21505-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ACTR5 antibody 21505-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21505-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21505-1-PBS cible Arp5/ACTR5 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Arp5/ACTR5 Protéine recombinante Ag15976
• Nom complet: ARP5 actin-related protein 5 homolog (yeast)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC038402
• Symbole du gène: ARP5/ACTR5
• Identification du gène (NCBI): 79913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

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