Anticorps Monoclonal anti-Arp5/ACTR5

• Phare
• Validé par KD/KO

Arp5/ACTR5 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E11C1

N° de cat : 67326-1-PBS

Synonymes

Actin related protein 5, ACTR5, Arp5, Arp5/ACTR5, FLJ12785, hARP5, INO80M, Sarcoma antigen NY SAR 16

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Galerie de données de validation

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  WB analysis using 67326-1-Ig (same clone as 67326-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67326-1-Ig (same clone as 67326-1-PBS)

  WB result of Arp5/ACTR5 antibody (67326-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Arp5/ACTR5 transfected HEK-293 cells. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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  WB analysis of C2C12 using 67326-1-Ig (same clone as 67326-1-PBS)

  C2C12 cells were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skeletal muscle using 67326-1-Ig (same clone as 67326-1-PBS)

  pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 67326-1-Ig (same clone as 67326-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67326-1-Ig (Arp5/ACTR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67326-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67326-1-PBS cible Arp5/ACTR5 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Arp5/ACTR5 Protéine recombinante Ag15976
• Nom complet: ARP5 actin-related protein 5 homolog (yeast)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC038402
• Symbole du gène: ARP5/ACTR5
• Identification du gène (NCBI): 79913
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Arp5 is a member of nuclear actin-related proteins (ARPs) that function as components of the chromatin- remodeling complex. Recently study reported that Arp5 regulates smooth muscle cell differentiation through interaction with myocardin (Myocd). Arp5 has been identified as negative regulator of Myocd, suppressing Myocd activity in the nucleus through tightly binding with Myocd RPEL motifs. Alternative splicing results in two variants of Arp5: a broadly expressed arp5-common, and a smooth muscle restricted arp5-sm. Expression of Arp5-sm protein is strongly suppressed in well-differentiated smooth muscle cells by partial messenger RNA decay and translational suppression. (24567363)

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