Anticorps Recombinant de lapin anti-Arrestin C

Arrestin C Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

242751B12

N° de cat : 85067-5-RR

Synonymes

Arrestin-C, ARR3, C-arrestin, CAR, ARRX

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Galerie de données de validation

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  WB analysis using 85067-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85067-5-RR (ARR3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 85067-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 85067-5-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Arrestin C antibody (85067-5-RR, Clone: 242751B12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85067-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85067-5-RR against Human Arrestin C were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, rat eye tissues
• Résultats positifs en IF-P: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

85067-5-RR cible Arrestin C dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Arrestin C Protéine recombinante Ag1580
• Nom complet: arrestin 3, retinal (X-arrestin)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC012096
• Symbole du gène: Arrestin C
• Identification du gène (NCBI): 407
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.

Publications

Species	Application	Title
human	IF	Sci Rep; The role of HMOX1-mediated ferroptosis in blue light-induced damage to retinal pigment epithelium; Authors - Chunyi Ji

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