Anticorps Polyclonal de lapin anti-Ataxin 2

Ataxin 2 Polyclonal Antibody for IHC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC

Conjugaison

Biotin

N° de cat : Biotin-21776

Synonymes

ataxin 2, ATX2, ATXN2, FLJ46772, SCA2, TNRC13



Applications testées

Résultats positifs en IHCtissu cérébral de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Immunohistochimie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-21776 cible Ataxin 2 dans les applications de IHC et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Ataxin 2 Protéine recombinante Ag16470
Nom complet ataxin 2
Masse moléculaire calculée 1313 aa, 140 kDa
Numéro d’acquisition GenBankBC114546
Symbole du gène ATXN2
Identification du gène (NCBI) 6311
Conjugaison Biotin
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

 ATXN2 (Ataxin-2) is a eukaryotic RNA‐binding protein that is conserved across species. It contains two LSm domains (RNA binding), a PAM2 motif (association with poly(A)-binding protein), and an N-terminal polyglutamine tract (PMID: 25027299). Ataxin-2 plays crucial roles at different stages of the regulation of posttranslational gene expression. ATXN2 is also directly implicated in the regulation of neural function by influencing specific molecular and cellular pathways. Genetic expansion of a poly‐glutamine tract in human ATXN2 has been linked to several neurodegenerative diseases, where it most likely acts through gain‐of‐function effects.

 What is the molecular weight of ATXN2? 

 The molecular weight varies from 12 to 140 kDa, depending on the isoform.

 What are the isoforms of ATXN2?

 ATXN2 has many isoforms, which differ significantly in length and domain composition.

 What is the subcellular localization of ATXN2?

 According to siRNA-based experiments, it is localized in the cytoplasmic compartment of normal cells, where it mostly associates with the Golgi apparatus and stress granules (PMID: 22508507).

 What is the tissue specificity of ATXN2?

 ATXN2 protein has been detected in various tissues; however, it is most highly expressed and studied in CNS. Significant amounts of this protein can also be detected in the liver and gallbladder.

 What is the molecular function of ATXN2?

 Ataxin-2 is involved in regulating various steps of mRNA translation, including poly‐A tailing, RNA stabilization, microRNA‐dependent gene silencing, and translational activation. All those functions are linked to its interactions with the poly(A)-binding protein. Ataxin-2 is involved in the formation of stress granules and P-bodies. Furthermore, genetic models of ATXN2 loss‐of‐function have underlined the importance of ATXN2 in mTOR signaling and cellular metabolism, which are all crucial for neural homeostasis (PMID: 29869836).

 What is ATXN2's involvement in disease?

 The N-terminal region of ATXN2 normally contains a polyQ stretch of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease) (PMID: 20740007). Long expansions of this tract result in spinocerebellar ataxia-2 (SCA2), an autosomal dominantly inherited, neurodegenerative disorder (PMID: 29427103). Genome-wide association studies indicate that loss-of-function mutations in this gene are associated with susceptibility to type I diabetes, obesity, and hypertension.


Protocole

Product Specific Protocols
IHC protocol for Biotin Ataxin 2 antibody Biotin-21776Download protocol
Standard Protocols
Click here to view our Standard Protocols