Anticorps Recombinant de lapin anti-Atox1

Atox1 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241566A7

N° de cat : 84305-1-PBS

Synonymes

241566A7, Atx1, HAH1

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Galerie de données de validation

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  WB analysis using 84305-1-RR (same clone as 84305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84305-1-RR (Atox1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01193-1

  Sandwich ELISA standard curve of MP01193-1, Mouse Atox1 Recombinant Matched Antibody Pair - PBS only. 84305-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35290. 84305-2-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  Sample test of MP01193-1

  The mean Atox1 concentration was determined to be 89.29 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  IHC staining of mouse heart using 84305-1-RR (same clone as 84305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84305-1-RR (Atox1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84305-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01193-1

  Cytometric bead array standard curve of MP01193-1, MOUSE Atox1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84305-1-PBS. Detection antibody: 84305-2-PBS. Standard: Ag35290. Range: 0.313-40 ng/mL.

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Informations sur le produit

84305-1-PBS cible Atox1 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Atox1 Protéine recombinante Ag35290
• Nom complet: ATX1 (antioxidant protein 1) homolog 1 (yeast)
• Masse moléculaire calculée: 7 kDa
• Poids moléculaire observé: 7 kDa
• Numéro d’acquisition GenBank: NM_009720
• Symbole du gène: Atox1
• Identification du gène (NCBI): 11927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.

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