Anticorps Recombinant de lapin anti-AXL

• Phare
• Validé par KD/KO

AXL Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242014B9

N° de cat : 84659-1-PBS

Synonymes

242014B9, EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

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Galerie de données de validation

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  WB analysis using 84659-1-RR (same clone as 84659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84659-1-RR (Axl antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 84659-1-RR (same clone as 84659-1-PBS)

  WB result of AXL antibody (84659-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AXL transfected HeLa cells. This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84659-1-RR (same clone as 84659-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AXL antibody (84659-1-RR, Clone: 242014B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84659-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84659-1-PBS cible AXL dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AXL Protéine recombinante Eg1618
• Nom complet: AXL receptor tyrosine kinase
• Masse moléculaire calculée: 98kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: NM_021913.4
• Symbole du gène: AXL
• Identification du gène (NCBI): 558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

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