Anticorps Polyclonal de lapin anti-Beta-2-Microglobulin

Beta-2-Microglobulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 13511-1-AP

Synonymes

B2M, Beta-2-microglobulin, HDCMA22P

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Galerie de données de validation

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  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13511-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 13511-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 13511-1-AP

  IP result of anti-Beta-2-microglobulin (IP:13511-1-AP, 4ug; Detection:13511-1-AP 1:600) with A431 cells lysate 2280 ug.

• 

  IHC staining of human tonsillitis using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13511-1-AP (Beta-2-microglobulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13511-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13511-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 13511-1-AP

  Immunofluorescent analysis of A431 cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:750 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NCCIT using 13511-1-AP

  Immunofluorescent analysis of NCCIT cells using Beta-2-Microglobulin antibody (13511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 13511-1-AP

  1X10^6 HeLa cells were stained with 0.20ug Beta-2-microglobulin antibody (13511-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules Jurkat, cellules Raji, tissu cardiaque humain, tissu d'estomac humain, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules A431,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de la prostate humain, tissu de cancer de l'œsophage humain, tissu de cancer du foie humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431, cellules NCCIT
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:375-1:1500
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

13511-1-AP cible Beta-2-Microglobulin dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta-2-Microglobulin Protéine recombinante Ag4433
• Nom complet: beta-2-microglobulin
• Masse moléculaire calculée: 119 aa, 14 kDa
• Poids moléculaire observé: 12-14 kDa
• Numéro d’acquisition GenBank: BC032589
• Symbole du gène: B2M
• Identification du gène (NCBI): 567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Publications

Species	Application	Title
human	IF	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
		Nat Commun; Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.; Authors - Kyota Fujita
mouse	WB	Theranostics; Nintedanib enhances the efficacy of PD-L1 blockade by upregulating MHC-I and PD-L1 expression in tumor cells.; Authors - Jingyao Tu
	IP	Biomater Sci; Enhanced β2-microglobulin binding of a "navigator" molecule bearing a single-chain variable fragment antibody for artificial switching of metabolic processing pathways.; Authors - Yusuke Kambe
rat	IHC	Aging (Albany NY); β2-microglobulin as a biomarker of pulmonary fibrosis development in COPD patients.; Authors - Zhenchao Wu
human,mouse	WB,IHC	Immunology; B2M gene expression shapes the immune landscape of lung adenocarcinoma and determines the response to immunotherapy.; Authors - Yu Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cali (Verified Customer) (10-24-2023)

A reliable Ab to detect endogenous B2M.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

Beta-2-Microglobulin Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:13511-1-AP)

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