Anticorps Recombinant de lapin anti-Beta-2-Microglobulin

Beta-2-Microglobulin Recombinant Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240660A12

N° de cat : 83683-6-PBS

Synonymes

B2M, 240660A12, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

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Galerie de données de validation

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  WB analysis using 83683-6-RR (same clone as 83683-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83683-6-PBS in a different storage buffer formulation.

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  IP experiment of A431 using 83683-6-RR (same clone as 83683-6-PBS)

  IP result of anti-Beta-2-Microglobulin (IP:83683-6-RR, 4ug; Detection:83683-6-RR 1:3000) with A431 cells lysate 2180 ug. This data was developed using the same antibody clone with 83683-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83683-6-RR (same clone as 83683-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83683-6-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83683-6-RR (same clone as 83683-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83683-6-RR (Beta-2-Microglobulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83683-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83683-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83683-6-RR against Human Beta-2-Microglobulin were performed. The affinity constant is 1.18 nM.

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Informations sur le produit

83683-6-PBS cible Beta-2-Microglobulin dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Beta-2-Microglobulin Protéine recombinante Ag4433
• Nom complet: beta-2-microglobulin
• Masse moléculaire calculée: 119 aa, 14 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC032589
• Symbole du gène: B2M
• Identification du gène (NCBI): 567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

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