Anticorps Monoclonal anti-B3GAT1

B3GAT1 Monoclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1F4A11

N° de cat : 66711-1-PBS

Synonymes

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

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Galerie de données de validation

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  WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 66711-1-Ig (same clone as 66711-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CD57 (66711-1-Ig, Clone:1F4A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66711-1-PBS cible B3GAT1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: B3GAT1 Protéine recombinante Ag13593
• Nom complet: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC010466
• Symbole du gène: B3GAT1
• Identification du gène (NCBI): 27087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.

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