Anticorps Polyclonal de lapin anti-B3GNT2

B3GNT2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14200-1-PBS

Synonymes

B3GALT7, b3Gal-T7, B3GNT1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7

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Galerie de données de validation

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  WB analysis using 14200-1-AP (same clone as 14200-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14200-1-AP (B3GNT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14200-1-AP (same clone as 14200-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14200-1-AP (B3GNT2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14200-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14200-1-PBS cible B3GNT2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B3GNT2 Protéine recombinante Ag5404
• Nom complet: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
• Masse moléculaire calculée: 9 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC030579
• Symbole du gène: B3GNT2
• Identification du gène (NCBI): 10678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

β-1,3-N-acetylglucosaminyltransferase (B3GNT2) is the most abundant and ubiquitously expressed B3GNT isoform and displays the greatest in vitro activity for N-glycan and O-glycan poly-LacNAc extensionis. B3GNT2 encodes a poly-N-acetyllactosamine synthase that targets >10 ligands and receptors to disrupt interactions between tumor and T cells and reduce T cell activation (PMID: 35338135, 32663509). B3GNT2 also has five predicted N-glycosylation sites at asparagine residues 79, 89, 127, 173, and 219 (PMID: 33229435). Western blots indicated the glycosylated form of B3GNT2 is detected at ~ 67 kDa.

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