Anticorps Polyclonal de lapin anti-B3GNT5

B3GNT5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 20422-1-AP

Synonymes

Beta3Gn-T5, beta3Gn T5, Beta-1,3-N-acetylglucosaminyltransferase 5, Beta-1,3-Gn-T5, Beta 1,3 Gn T5

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Galerie de données de validation

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  WB analysis of mouse lung using 20422-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20422-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20422-1-AP (B3GNT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 20422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20422-1-AP (B3GNT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20422-1-AP (B3GNT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules MCF-7
• Résultats positifs en IHC: tissu pulmonaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

20422-1-AP cible B3GNT5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B3GNT5 Protéine recombinante Ag14246
• Nom complet: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
• Masse moléculaire calculée: 378 aa, 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC028058
• Symbole du gène: B3GNT5
• Identification du gène (NCBI): 84002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

B3GNT5 (UDP-GlcNAc:β-Gal β-1,3-N-acetylglucosaminyltransferase 5) is a glycosyltransferase that transfers an N-acetylglucosamine (GlcNAc) from uridine diphosphate (UDP)-GlcNAc to galactose at the nonreducing end of carbohydrate chain. B3GNT5 is required for tumorigenicity of breast cancer and glycosylation of B3GNT5 is critical for its protein stabilization (PMID: 35526049). B3GNT5 was highly expressed in human GBM and glioma cell lines in culture, and was associated with poor patient survival (PMID: 32677340). B3GNT5 was N-glycosylated, and glycosylation of B3GNT5 stabilized its protein for non-glycosylated B3GNT5 protein degraded more rapidly than glycosylated form (PMID: 35526049).

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; MiR-30a-5p inhibits proliferation and metastasis of hydatidiform mole by regulating B3GNT5 through ERK/AKT pathways.; Authors - Zhenzhen Guo
human	WB	Cell Rep; Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins; Authors - Jicheng Yang
human	WB,IHC	iScience; B3GNT5 is a novel marker correlated with malignant phenotype and poor outcome in pancreatic cancer; Authors - Wei Yao ; KD Validated

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