Anticorps Polyclonal de lapin anti-B3GNT6

B3GNT6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21291-1-PBS

Synonymes

BGnT-6, BGnT 6, Beta-1,3-N-acetylglucosaminyltransferase 6, B3Gn-T6, B3Gn T6

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Galerie de données de validation

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  WB analysis using 21291-1-AP (same clone as 21291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21291-1-AP (B3GNT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21291-1-AP (same clone as 21291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21291-1-AP (B3GNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21291-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21291-1-PBS cible B3GNT6 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B3GNT6 Protéine recombinante Ag15825
• Nom complet: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
• Masse moléculaire calculée: 384 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC103910
• Symbole du gène: B3GNT6
• Identification du gène (NCBI): 192134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The B3GNT6 protein, also known as core 3 synthase, is a member of the O-GlcNAc transferase (OGT) family and adds an N-acetylglucosamine to N-acetylgalactosamine-modifed serine or threonine. The B3GNT6 protein is responsible for the formation of the core 3 structure of O-glycans, which are important components of mucin-type glycoproteins. This process is known as the protein O-GlcNAcylation modifcation. B3GNT6 expression has been reported to be largely downregulated in gastric and colorectal cancers. B3GNT6 has 3 isoforms with the molecular mass of 29 and 43 kDa. (PMID: 35387659)

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