Anticorps Polyclonal de lapin anti-B4GALT2

B4GALT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 20330-1-AP

Synonymes

B4Gal T2, B4Gal T3, B4GALT2, Beta 1,4 GalTase 2, beta4Gal T2

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Galerie de données de validation

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  WB analysis of PC-3 using 20330-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse large intestine using 20330-1-AP

  mouse large intestine tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20330-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 20330-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20330-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20330-1-AP (B4GALT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 20330-1-AP (B4GALT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 20330-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20330-1-AP (B4GALT2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, tissu cérébral de souris, tissu de gros intestin de souris, tissu de muscle squelettique de souris, tissu ovarien de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

20330-1-AP cible B4GALT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B4GALT2 Protéine recombinante Ag14046
• Nom complet: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2
• Masse moléculaire calculée: 372 aa, 42 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC002431
• Symbole du gène: B4GALT2
• Identification du gène (NCBI): 8704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Genet; Identification of a Novel Glycosyltransferase Prognostic Signature in Hepatocellular Carcinoma Based on LASSO Algorithm.; Authors - Zhiyang Zhou
human	IHC	J Immunother Cancer; Novel post-translational modification learning signature reveals B4GALT2 as an immune exclusion regulator in lung adenocarcinoma; Authors - Pengpeng Zhang

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