Anticorps Polyclonal de lapin anti-B4GALT3

B4GALT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11041-1-PBS

Synonymes

b4Gal T3, b4Gal-T3, Beta 1,4 GalTase 3, Beta-1,4-galactosyltransferase 3, Beta-1,4-GalTase 3

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Galerie de données de validation

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  WB analysis using 11041-1-AP (same clone as 11041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11041-1-AP (B4GALT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11041-1-AP (same clone as 11041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11041-1-AP (B4GALT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 11041-1-AP (B4GALT3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 11041-1-AP (same clone as 11041-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using B4GALT3 antibody (11041-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11041-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11041-1-PBS cible B4GALT3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B4GALT3 Protéine recombinante Ag1487
• Nom complet: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC009985
• Symbole du gène: B4GALT3
• Identification du gène (NCBI): 8703
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

B4GALT3 is an enzyme responsible for the generation of poly-N-acetyllactosamine. The B4GALTs transfer galactose from UDP-Gal to N-acetylglucosamine (GlcNAc)-terminated oligosaccharides on N-glycan, O-glycan, or glycolipid to form N-acetyllactosamin (PMID: 23444218). B4GALT3 has been found to regulate cancer cell invasion (PMID: 25659296). B4GALT3 knockdown increased cell migration, invasion and the activation of β1 integrin and its downstream signaling (PMID: 24403309).

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