Anticorps Polyclonal de lapin anti-CD80/B7-1

CD80/B7-1 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 14292-1-AP

Synonymes

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

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Galerie de données de validation

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  WB analysis using 14292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood leukocyte using 14292-1-AP

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 14292-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of Raji using 14292-1-AP

  1X10^6 Raji cells were stained with 0.2ug CD80/B7-1 antibody (14292-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Ramos, cellules RAW 264.7, leucocyte de sang périphérique humain
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• FC: See 8 publications below

Informations sur le produit

14292-1-AP cible CD80/B7-1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD80/B7-1 Protéine recombinante Ag5615
• Nom complet: CD80 molecule
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC042665
• Symbole du gène: CD80
• Identification du gène (NCBI): 941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	IHC	ACS Appl Mater Interfaces; Fe(III)-Chelated Polydopamine Nanoparticles for Synergistic Tumor Therapies of Enhanced Photothermal Ablation and Antitumor Immune Activation.; Authors - Na Xu
mouse	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
mouse	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
mouse		Int J Nanomedicine; Self-Assembly Catalase Nanocomplex Conveyed by Bacterial Vesicles for Oxygenated Photodynamic Therapy and Tumor Immunotherapy.; Authors - Jiayu Zhang
mouse	IHC	J Nanobiotechnology; Continuous ZnO nanoparticle exposure induces melanoma-like skin lesions in epidermal barrier dysfunction model mice through anti-apoptotic effects mediated by the oxidative stress-activated NF-κB pathway.; Authors - Ping Wang
mouse	FC	Drug Deliv; Sonosensitizer nanoplatform-mediated sonodynamic therapy induced immunogenic cell death and tumor immune microenvironment variation.; Authors - Jing Zheng

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