Anticorps Monoclonal anti-CD80

CD80 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (2)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(73)

CloneNo.

1E2F10

N° de cat : 66406-1-Ig

Synonymes

1E2F10, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1

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Galerie de données de validation

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  WB analysis of Raji using 66406-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DC2.4 using 66406-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66406-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules DC2.4

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IF: See 24 publications below

Informations sur le produit

66406-1-Ig cible CD80 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, Lapin, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD80 Protéine recombinante Ag5615
• Nom complet: CD80 molecule
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC042665
• Symbole du gène: CD80
• Identification du gène (NCBI): 941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse		Acta Pharm Sin B; Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy; Authors - Lina Guo
human	IF	Cell Rep Med; Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers; Authors - Bingxin Liu
mouse	WB	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
		J Nanobiotechnology; Dual-modality immune nano-activator harnessing Mn2⁺ and quercetin to potentiate the cGAS-STING pathway for advanced cancer metalloimmunotherapy; Authors - Shanshan Ma
mouse,human		Theranostics; ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.; Authors - Xiaozheng Chen
human	WB	Int J Biol Macromol; Study on the mechanism of macrophages activated by phosphoesterified rehmanniae polysaccharide on human gastric cancer cells; Authors - Xianglong Liu

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