Anticorps Polyclonal de lapin anti-CD86

CD86 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 30691-1-AP

Synonymes

B7, B7 2, B7.2, B70, B7-2

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Galerie de données de validation

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  WB analysis using 30691-1-AP

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30691-1-AP (CD86 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules DC2.4, cellules RAW 264.7, tissu splénique de rat, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Informations sur le produit

30691-1-AP cible CD86 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD86 Protéine recombinante Ag33514
• Nom complet: CD86 antigen
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_019388
• Symbole du gène: Cd86
• Identification du gène (NCBI): 12524
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	IF	J Inflamm Res; B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue; Authors - Peizhi Lu
mouse	WB	J Control Release; Reprogramming tumor-associated macrophages by a dually targeted milk exosome system as a potent monotherapy for cancer; Authors - Ying Chen
rat,mouse	WB,IF	Brain Res Bull; Isobavachalcone alleviates ischemic stroke by suppressing HDAC1 expression and improving M2 polarization; Authors - Qiannan Zhang
mouse	WB	Adv Healthc Mater; Legumain-Guided Ferulate-Peptide Self-Assembly Enhances Macrophage-Endotheliocyte Partnership to Promote Therapeutic Angiogenesis After Myocardial Infarction; Authors - Delong Xu
mouse	WB	Inflammation; Protective Effects of a Dihydrodiazepine Against Endotoxin Shock Through Suppression of TLR4/NF-κB/IRF3 Signaling Pathways; Authors - Hamza Hanieh
rat,mouse	IHC,IF	Turk J Med Sci; Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats; Authors - Bo Ke

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