Anticorps Recombinant de lapin anti-CD86

CD86 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250726D3

N° de cat : 86252-1-PBS

Synonymes

B7 2, B7-2, CD86 molecule

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Galerie de données de validation

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  WB analysis of rat spleen using 86252-1-RR (same clone as 86252-1-PBS)

  rat spleen tissue was subjected to SDS PAGE followed by western blot with 86252-1-RR (CD86 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86252-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP02379-1

  The mean CD86 concentration was determined to be 105.1 ng/mL in Rat spleen tissue extract based on a 8.8 mg/mL extract load.

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  Cytometric bead array standard curve of MP02379-1

  Cytometric bead array standard curve of MP02379-1, RAT CD86 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86252-2-PBS. Detection antibody: 86252-1-PBS. Standard: Eg3586. Range: 0.312-40 ng/mL.

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Informations sur le produit

86252-1-PBS cible CD86 dans les applications de WB, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD86 Protéine recombinante Eg3586
• Nom complet: CD86 molecule
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: NM_020081.1
• Symbole du gène: Cd86
• Identification du gène (NCBI): 56822
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

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