Anticorps Recombinant de lapin anti-ICOSLG/B7-H2/CD275

ICOSLG/B7-H2/CD275 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240243G5

N° de cat : 83375-2-RR

Synonymes

B7-H2/ICOSLG, B7-H2, CD275, Icosl, GL50

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Galerie de données de validation

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  WB analysis using 83375-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 83375-2-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83375-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83375-2-RR (B7-H2/ICOSLG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83375-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83375-2-RR against Mouse B7-H2/ICOSLG were performed. The affinity constant is 0.292 nM.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83375-2-RR cible ICOSLG/B7-H2/CD275 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: icos ligand
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: NM_015790.3
• Symbole du gène: CD275
• Identification du gène (NCBI): 50723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

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