Anticorps Polyclonal de lapin anti-B7‑H4

B7‑H4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12080-1-PBS

Synonymes

VTCN1, B7S1, B7-H4, B7H4, B7h.5

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Galerie de données de validation

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  WB analysis of mouse liver using 12080-1-AP (same clone as 12080-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 12080-1-AP (same clone as 12080-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 12080-1-AP (same clone as 12080-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12080-1-AP (B7‑H4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 12080-1-AP (same clone as 12080-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12080-1-AP (B7‑H4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12080-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12080-1-PBS cible B7‑H4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: B7‑H4 Protéine recombinante Ag2712
• Nom complet: V-set domain containing T cell activation inhibitor 1
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 55-65 kDa
• Numéro d’acquisition GenBank: BC065717
• Symbole du gène: B7-H4
• Identification du gène (NCBI): 79679
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

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