Anticorps Monoclonal anti-B7‑H4

B7‑H4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5B1

N° de cat : 66817-1-PBS

Synonymes

VTCN1, B7-H4, B7H4, B7h.5, B7 homolog 4

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Galerie de données de validation

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  WB analysis using 66817-1-Ig (same clone as 66817-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66817-1-Ig (B7‑H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66817-1-Ig (same clone as 66817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66817-1-Ig (same clone as 66817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 66817-1-Ig (same clone as 66817-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66817-1-Ig (B7‑H4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66817-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66817-1-PBS cible B7‑H4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: B7‑H4 Protéine recombinante Ag27751
• Nom complet: V-set domain containing T cell activation inhibitor 1
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC065717
• Symbole du gène: B7-H4
• Identification du gène (NCBI): 79679
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

B7‑H4 also named VTCN1, B7X, or B7S1 is a 282 amino acid protein, which contains 2 immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7‑H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7‑H4 is a single-pass type I membrane protein, which is over-expressed in breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

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