Anticorps Recombinant de lapin anti-B7-H4

B7-H4 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250392C3

N° de cat : 86484-1-PBS

Synonymes

B7H4, B7S1, B7x, V-set domain-containing T-cell activation inhibitor 1, Vtcn1

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Galerie de données de validation

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  WB analysis using 86484-1-RR (same clone as 86484-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86484-1-RR (B7-H4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86484-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02632-1

  The mean B7-H4 concentration was determined to be 23.71 ng/mL in rat liver extract based on a 8.20 mg/mL extract load and 16.72 ng/mL in mouse uterus extract based on a 3.40 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02632-1

  Sandwich ELISA standard curve of MP02632-1, Rat B7-H4 Recombinant Matched Antibody Pair - PBS only. 86484-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3230. 86484-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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Informations sur le produit

86484-1-PBS cible B7-H4 dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: B7-H4 Protéine recombinante Eg3230
• Nom complet: V-set domain containing T cell activation inhibitor 1
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: NM_001024244.1
• Symbole du gène: Vtcn1
• Identification du gène (NCBI): 295322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

B7-H4, also named VTCN1, B7X, or B7S1, is a single-pass type I membrane protein, which contains two immunoglobulin-like domains and belongs to the immunoglobulin superfamily. B7-H4 negatively regulates T-cell mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. B7-H4 is over-expressed in many cancers, including breast, ovarian, endometrial, renal cell and non-small-cell lung cancers. The predicted molecular weight of B7‑H4 is 31 kDa. The glycosylated B7‑H4 is 50 to 80 kDa, and the non-glycosylated form is 28 kDa.

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