Anticorps Recombinant de lapin anti-B7-H6

• Phare
• Validé par KD/KO

B7-H6 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240808F7

N° de cat : 83908-3-RR

Synonymes

B7H6, NCR3LG1, 240808F7, B7 H6, B7 homolog 6

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Galerie de données de validation

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  WB analysis of HEK-293 using 83908-3-RR

  WB result of B7-H6 antibody (83908-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B7-H6 transfected HEK-293 cells.

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  WB analysis using 83908-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83908-3-RR (B7H6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83908-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83908-3-RR against Human B7-H6 were performed. The affinity constant is 2.38 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HeLa, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83908-3-RR cible B7-H6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: B7-H6 Protéine recombinante Eg0007
• Nom complet: hypothetical protein DKFZp686O24166
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC136800
• Symbole du gène: B7H6
• Identification du gène (NCBI): 374383
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

B7-H6, also known as NCR3LG1, is a member of the B7 family of immunoreceptors. It is a 454-aa-long type I transmembrane protein consisting of an extracellular region, a transmembrane segment, and a cytoplasmic domain which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), an SH2-binding domain, and an SH3-binding motif. B7-H6 triggers NKp30-mediated activation of human NK cells (PMID: 19528259). It has been reported that B7H6 is expressed in various types of tumor cells and plays a prominent role in natural killer cell immunosurveillance (PMID: 35697295). The apparent molecular weight of B7-H6 is larger than the calculated molecular weight of 51 kDa due to glycosylation (PMID: 19528259).

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