Anticorps Monoclonal anti-BACH1

• Phare
• Validé par KD/KO

BACH1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D12A4

N° de cat : 66762-1-PBS

Synonymes

2D12A4, BACH 1, BACH-1

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Galerie de données de validation

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  WB analysis using 66762-1-Ig (same clone as 66762-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66762-1-Ig (same clone as 66762-1-PBS)

  WB result of BACH1 antibody (66762-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66762-1-Ig (same clone as 66762-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66762-1-Ig (same clone as 66762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66762-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50384-1

  Cytometric bead array standard curve of MP50384-1, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-2-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

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  Cytometric bead array standard curve of MP50384-2

  Cytometric bead array standard curve of MP50384-2, BACH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 66762-3-PBS. Standard:Ag6011. Range: 6.25-200 ng/mL.

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  Cytometric bead array standard curve of MP80007-3

  Cytometric bead array standard curve of MP80007-3, BACH1 Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 66762-1-PBS. Detection antibody: 82842-2-PBS. Standard: Ag6011. Range: 0.313-40 ng/mL.

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Informations sur le produit

66762-1-PBS cible BACH1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BACH1 Protéine recombinante Ag6011
• Nom complet: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC063307
• Symbole du gène: BACH1
• Identification du gène (NCBI): 571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

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