Anticorps Recombinant de lapin anti-BACH1

BACH1 Recombinant Antibody for WB, IF/ICC, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat

Applications

WB, IF/ICC, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

4K14

Avis (1)

N° de cat : 82842-7-RR

Synonymes

4K14, BACH 1, BACH-1

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Galerie de données de validation

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  WB analysis using 82842-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82842-7-RR (BACH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82842-7-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BACH1 antibody (82842-7-RR, Clone: 4K14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  ChIP experiment of K-562 using 82842-7-RR

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of BACH1 (82842-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562, cellules Raji, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en ChIP-qPCR: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82842-7-RR cible BACH1 dans les applications de WB, IF/ICC, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BACH1 Protéine recombinante Ag5128
• Nom complet: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Masse moléculaire calculée: 82 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC063307
• Symbole du gène: BACH1
• Identification du gène (NCBI): 571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Agnese (Verified Customer) (07-03-2025)

The Ab seem to work fine in our sample type (hMDM) and application (immunofluorescence and PLA).

• Applications: Immunofluorescence, Other
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human monocyte-derived macrophages (hMDM)

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