Anticorps Polyclonal de lapin anti-BACH2

• Phare
• Validé par KD/KO

BACH2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27635-1-AP

Synonymes

BACH2, BTB and CNC homolog 2

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Galerie de données de validation

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  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 27635-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 27635-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 27635-1-AP (BACH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Ramos

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

27635-1-AP cible BACH2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BACH2 Protéine recombinante Ag26526
• Nom complet: BTB and CNC homology 1, basic leucine zipper transcription factor 2
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_001170794
• Symbole du gène: BACH2
• Identification du gène (NCBI): 60468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BACH2, also named as Transcription regulator protein BACH2, is a 841 amino acid protein, which is widely expressed within the B-lymphoid lineage, except in plasma cells. BACH2 contains an N-terminal BTB domain involved in its transcriptional function, but instead of having zinc fingers at the C-terminus, it binds to DNA through a basic leucine zipper motif. BACH2 binds DNA consensus sequences, termed MARE motifs, by forming a heterodimer with small leucine zipper MAF proteins such as MAFK, MAFG, and MAFF. The calculated molecular weight of BACH2 is 92 kDa, but the post-modifiction of BACH2 is about 120-130 kDa. (PMID: 24277074 )

Publications

Species	Application	Title
human	WB	FEBS Open Bio; Bach2 overexpression represses Th9 cell differentiation by suppressing IRF4 expression in systemic lupus erythematosus.; Authors - Yujun Sheng ; KD Validated
human	WB	Cell Rep; Class I HDAC inhibitors enhance antitumor efficacy and persistence of CAR-T cells by activation of the Wnt pathway; Authors - Meng Zhu
human	WB,IHC	World J Surg Oncol; Prognostic value of circadian rhythm-associated genes in breast cancer; Authors - Ling Wang

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