Anticorps Monoclonal anti-BAD
BAD Monoclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
WB, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
4B12G2
N° de cat : 67830-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules Raji, cellules RAW 264.7, cellules THP-1 | 
| Résultats positifs en IF/ICC | cellules HeLa, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 9 publications below | 
Informations sur le produit
67830-1-Ig cible BAD dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris | 
| Réactivité citée | rat, Humain, souris | 
| Hôte / Isotype | Mouse / IgG1 | 
| Clonalité | Monoclonal | 
| Type | Anticorps | 
| Immunogène | Peptide | 
| Nom complet | BCL2-associated agonist of cell death | 
| Masse moléculaire calculée | 18 kDa | 
| Poids moléculaire observé | 18-25 kDa | 
| Numéro d’acquisition GenBank | BC001901 | 
| Symbole du gène | BAD | 
| Identification du gène (NCBI) | 572 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par protéine G | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for BAD antibody 67830-1-Ig | Download protocol | 
| IF protocol for BAD antibody 67830-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Adv Sci (Weinh) Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy | ||
| Oxid Med Cell Longev Curcumin Inhibits Hyperandrogen-Induced IRE1α-XBP1 Pathway Activation by Activating the PI3K/AKT Signaling in Ovarian Granulosa Cells of PCOS Model Rats | ||
| FASEB J Glucose-dependent insulinotropic polypeptide modifies adipose plasticity and promotes beige adipogenesis of human omental adipose-derived stem cells. | ||
| Phytomedicine Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity | ||
| J Transl Med circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA | ||
| Toxicol Appl Pharmacol RGS2 attenuates alveolar macrophage damage by inhibiting the Gq/11-Ca2+ pathway during cowshed PM2.5 exposure, and aberrant RGS2 expression is associated with TLR2/4 activation | 


