Anticorps Monoclonal anti-BAD

BAD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4B12G2

N° de cat : 67830-1-PBS

Synonymes

4B12G2, BBC2, BBC6, Bcl2-associated agonist of cell death, Bcl-2-binding component 6

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Galerie de données de validation

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  WB analysis using 67830-1-Ig (same clone as 67830-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 67830-1-Ig (same clone as 67830-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67830-1-Ig (BAD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67830-1-Ig (same clone as 67830-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAD antibody (67830-1-Ig, Clone: 4B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67830-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67830-1-PBS cible BAD dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: BCL2-associated agonist of cell death
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18-25 kDa
• Numéro d’acquisition GenBank: BC001901
• Symbole du gène: BAD
• Identification du gène (NCBI): 572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

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