Anticorps Recombinant de lapin anti-BAF170

BAF170 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251330D11

N° de cat : 86473-3-RR

Synonymes

SMARCC2, BRG1-associated factor 170, CRACC2, Rsc8, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2

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Galerie de données de validation

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  WB analysis using 86473-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86473-3-RR (BAF170 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86473-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86473-3-RR against Human BAF170 were performed. The affinity constant is 4.64 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules C6, cellules HeLa, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86473-3-RR cible BAF170 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BAF170 Protéine recombinante Ag2634
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
• Masse moléculaire calculée: 1214 aa, 132 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC013045
• Symbole du gène: BAF170
• Identification du gène (NCBI): 6601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)

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