Anticorps Recombinant de lapin anti-BAFF

BAFF Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242006E4

N° de cat : 84541-3-RR

Synonymes

242006E4

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Galerie de données de validation

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  WB analysis using 84541-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 84541-3-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 84541-3-RR (BAFF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules Jurkat, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84541-3-RR cible BAFF dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FusionProtein
• Nom complet: tumor necrosis factor (ligand) superfamily, member 13b
• Masse moléculaire calculée: 34kd
• Poids moléculaire observé: 32kd, 17kd
• Numéro d’acquisition GenBank: NM_033622
• Symbole du gène: Tnfsf13b
• Identification du gène (NCBI): 24099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAFF (B-cell-activating factor), also called Tnfsf13b (Tumor necrosis factor ligand superfamily member 13B) and CD257, is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in stimulating B- and T-cell function and the regulation of humoral immunity. A third B-cell-specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response (PMID: 12867412). BAFF cleaved into 2 chains: membrane form is 32-35 kDa; soluble form is 17 kDa.

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