Anticorps Recombinant de lapin anti-BAG2

BAG2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242717G4

N° de cat : 85063-4-RR

Synonymes

242717G4, BAG-2, KIAA0576

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Galerie de données de validation

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  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85063-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85063-4-RR (BAG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85063-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85063-4-RR against Human BAG2 were performed. The affinity constant is 0.644 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MG U-87, cellules U-251, tissu testiculaire de rat, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85063-4-RR cible BAG2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BAG2 Protéine recombinante Ag30873
• Nom complet: BCL2-associated athanogene 2
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NM_004282
• Symbole du gène: BAG2
• Identification du gène (NCBI): 9532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).

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