Anticorps Polyclonal de lapin anti-BAG3

• Phare
• Validé par KD/KO

BAG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10599-1-PBS

Synonymes

BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, BIS, Docking protein CAIR-1

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Galerie de données de validation

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  WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  WB analysis using 10599-1-AP (same clone as 10599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 10599-1-AP (same clone as 10599-1-PBS)

  IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug. This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10599-1-AP (same clone as 10599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10599-1-AP (same clone as 10599-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10599-1-AP (BAG3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BAG3 antibody (10599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10599-1-AP (same clone as 10599-1-PBS)

  Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10599-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10599-1-PBS cible BAG3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAG3 Protéine recombinante Ag0956
• Nom complet: BCL2-associated athanogene 3
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 74-80 kDa
• Numéro d’acquisition GenBank: BC006418
• Symbole du gène: BAG3
• Identification du gène (NCBI): 9531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.

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