Anticorps Monoclonal anti-BAG3

BAG3 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

4G6C2

N° de cat : 68076-1-Ig

Synonymes

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

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Galerie de données de validation

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  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68076-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68076-1-Ig (BAG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68076-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using BAG3 antibody (68076-1-Ig, Clone: 4G6C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, cellules LNCaP, cœur de porc, cœur de rat, tissu cardiaque de lapin
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

68076-1-Ig cible BAG3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BAG3 Protéine recombinante Ag17608
• Nom complet: BCL2-associated athanogene 3
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC006418
• Symbole du gène: BAG3
• Identification du gène (NCBI): 9531
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy.

Publications

Species	Application	Title
human,rat	IHC	J Physiol Biochem; BAG3 protects chondrocytes against lumbar facet joint osteoarthritis by regulating autophagy and apoptosis.; Authors - Xin Lu
rat	WB,IF	J Physiol Sci; High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle; Authors - Jiao Lu