Anticorps Polyclonal de lapin anti-SODD/BAG4

SODD/BAG4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13913-1-AP

Synonymes

SODD, BAG4, BAG 4, Silencer of death domains, Bcl-2-associated athanogene 4

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Galerie de données de validation

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  WB analysis of K-562 using 13913-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13913-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13913-1-AP (SODD/BAG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13913-1-AP

  IP result of anti-SODD/BAG4 (IP:13913-1-AP, 4ug; Detection:13913-1-AP 1:500) with K-562 cells lysate 2800ug.

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  IF Staining of MCF-7 using 13913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SODD/BAG4 antibody (13913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13913-1-AP cible SODD/BAG4 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SODD/BAG4 Protéine recombinante Ag4905
• Nom complet: BCL2-associated athanogene 4
• Masse moléculaire calculée: 457 aa, 50 kDa
• Poids moléculaire observé: 60 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: BC038505
• Symbole du gène: SODD/BAG4
• Identification du gène (NCBI): 9530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG4, also known as SODD (the silencer of death domain), belongs to the BAG family of anti-apoptotic proteins. BAG4 binds to the tumor necrosis factor receptor 1 (TNFR1) and block signaling for subsequent activation of apoptosis. This antibody detects 66-70 kDa full-length BAG4 protein and 45 kDa, 60 kDa C-terminal fragments, which was also observed by Hedda et al. (12706861).

Publications

Species	Application	Title
Artemia sinica	WB	Gene; Identification, expression pattern and functional characterization of As-kip2 in diapause embryo restarting process of Artemia sinica.; Authors - Mengchen Zhang
	WB	Nat Commun; Mettl3-dependent m6A modification attenuates the brain stress response in Drosophila; Authors - Alexandra E. Perlegos
human	WB,IF,CoIP	Mol Med; USP14 inhibits mitophagy and promotes tumorigenesis and chemosensitivity through deubiquitinating BAG4 in microsatellite instability-high colorectal cancer; Authors - Zhiyong Wang

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