Anticorps Polyclonal de lapin anti-BAHCC1

BAHCC1 Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 30046-1-AP

Synonymes

BAHCC1, BAHD2, FLJ23058, KIAA1447

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Galerie de données de validation

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  IHC staining of mouse brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 30046-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 30046-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAHCC1 antibody (30046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30046-1-AP cible BAHCC1 dans les applications de IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAHCC1 Protéine recombinante Ag32599
• Nom complet: BAH domain and coiled-coil containing 1
• Masse moléculaire calculée: 280 kDa
• Poids moléculaire observé: 280 kDa
• Numéro d’acquisition GenBank: NM_001291324.1
• Symbole du gène: BAHCC1
• Identification du gène (NCBI): 57597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAHCC1 (BAH and coiled-coil domain-containing protein 1) is also named as BAHD2, KIAA1447 and BAH domain-containing protein 2. BAHCC1 is a transcriptional regulator controlling expression of E2F/KLF-dependent cell-cycle and DNA-repair genes. BAHCC1 associates with BRG1-containing remodeling complexes at the promoters of these genes. BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair. Consequently, BAHCC1 deficiency cooperates with PARP inhibition to induce melanoma cell death (PMID: 37924516). depletion of BAHCC1, or disruption of the BAHCC1BAH-H3K27me3 interaction, causes derepression of H3K27me3-targeted genes that are involved in tumor suppression and cell differentiation, leading to suppression of oncogenesis (PMID: 33139953).