Anticorps Polyclonal de lapin anti-IRSp53

IRSp53 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11087-2-AP

Synonymes

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSp53, IRSp53/58, Protein BAP2

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Galerie de données de validation

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  WB analysis of mouse brain using 11087-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11087-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11087-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11087-2-AP (IRSp53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11087-2-AP

  IP Result of anti-IRSp53 (IP:11087-2-AP, 4ug; Detection:11087-2-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of mouse brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11087-2-AP (IRSp53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11087-2-AP (IRSp53 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 11087-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11087-2-AP (IRSp53 antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11087-2-AP cible IRSp53 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRSp53 Protéine recombinante Ag1552
• Nom complet: BAI1-associated protein 2
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC014020
• Symbole du gène: BAIAP2
• Identification du gène (NCBI): 10458
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	WB	Biol Cell; Extracellular vesicles containing the I-BAR protein IRSp53 are released from the cell plasma membrane in an Arp2/3 dependent manner.; Authors - Alice Trausch
hamster	WB,IP	Proteomics; Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell-Cell Transmission.; Authors - Fei-Long Yu
mouse	WB	JCI Insight; Disruption of Robo2-Baiap2 integrated signaling drives cystic disease.; Authors - Qinggang Li
mouse	IF	Lab Invest; FAK Signaling Induces the Onset of Proteinuria via FAK Signaling in a Mouse Model of Lung Cancer; Authors - Sheng-Wen Niu
human	IHC	Elife; Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes; Authors - Aojia Zhuang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

anwesh (Verified Customer) (10-28-2019)

Bands detected at the correct molecular weight. Great antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, U2OS, A549

IRSp53 Antibody Western Blot, validation (1:1000 dilution) in 293T, U2OS, A549 (Cat no:11087-2-AP)

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