Anticorps Monoclonal anti-IRSp53

IRSp53 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1D4G1

N° de cat : 68348-1-Ig

Synonymes

BAI associated protein 2, BAI1 associated protein 2, BAIAP2, BAP2, Fas ligand associated factor 3, FLAF3, Insulin receptor substrate p53, IRS 58, IRSP53, IRSp53/58, Protein BAP2

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Galerie de données de validation

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  WB analysis using 68348-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68348-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit cerebellum using 68348-1-Ig

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68348-1-Ig (IRSp53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de lapin, cellules LNCaP, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de lapin

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68348-1-Ig cible IRSp53 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IRSp53 Protéine recombinante Ag25161
• Nom complet: BAI1-associated protein 2
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC014020
• Symbole du gène: IRSp53
• Identification du gène (NCBI): 10458
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRSp53 (also known as BAIAP2) is a multi-domain adaptor protein that originally identified as a 58/53-kDa substrate of insulin-receptor kinase in the hamster (PMID: 10332026). As a BAI1-binding protein, IRSp53 plays an important role in linking between membrane and cytoskeleton in the process of neuronal growth (PMID: 10343108). IRSp53 is necessary for Cdc42-mediated reorganization of the actin cytoskeleton and for Rac1-mediated membrane ruffling. Alternatively spliced isoforms of IRSp53 exist. IRSp53 has been implicated in several psychiatric disorders, including autism spectrum disorders (ASDs), schizophrenia, and attention deficit/hyperactivity disorder (ADHD) (PMID: 26275848).

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