Anticorps Polyclonal de lapin anti-BAK

• Phare
• Validé par KD/KO

BAK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29552-1-PBS

Synonymes

BAK1, Bcl-2 homologous antagonist/killer, Bcl2-L-7, Bcl-2-like protein 7, CDN1

---

Galerie de données de validation

• 

  WB analysis using 29552-1-AP (same clone as 29552-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29552-1-AP (BAK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29552-1-AP (same clone as 29552-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29552-1-AP (BAK antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 29552-1-AP (same clone as 29552-1-PBS)

  WB result of BAK antibody (29552-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAK transfected A431 cells. This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A431 using 29552-1-AP (same clone as 29552-1-PBS)

  IP result of anti-BAK (IP:29552-1-AP, 4ug; Detection:29552-1-AP 1:3000) with A431 cells lysate 1040 ug. This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29552-1-AP (same clone as 29552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29552-1-AP (BAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29552-1-AP (same clone as 29552-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29552-1-AP (BAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 29552-1-AP (same clone as 29552-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BAK antibody (29552-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29552-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

29552-1-PBS cible BAK dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAK Protéine recombinante Ag31042
• Nom complet: BCL2-antagonist/killer 1
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: NM_001188
• Symbole du gène: BAK1
• Identification du gène (NCBI): 578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

{{ptg:RelatedPrimaryAntibodies}}