Anticorps Polyclonal de lapin anti-BAP1

BAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10398-1-AP

Synonymes

BAP1, BRCA1 associated protein 1, Cerebral protein 6, HUCEP 13, hucep 6, KIAA0272, UCHL2

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Galerie de données de validation

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  WB analysis of A2780 using 10398-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10398-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10398-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10398-1-AP (BAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10398-1-AP

  IP result of anti-BAP1 (IP:10398-1-AP, 4ug; Detection:10398-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10398-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10398-1-AP (BAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A2780, tissu ovarien de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

10398-1-AP cible BAP1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAP1 Protéine recombinante Ag0597
• Nom complet: BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase)
• Masse moléculaire calculée: 81-91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC001596
• Symbole du gène: BAP1
• Identification du gène (NCBI): 8314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRCA1-associated protein-1 (BAP1),also named as KIAA0272, hucep-6, DKFZp686N04275, FLJ35406, FLJ37180, HUCEP-13, is a deubiquitinating enzyme whose function in the control of the cell cycle requires both its deubiquitinating activity and nuclear localization(PMID:22374320). It helps to control cell proliferation by regulating HCFC1 protein levels and by associating with genes involved in the G(1)-S transition)(PMID:19188440). Defects in BAP1 may be a cause of mesothelioma malignant (MESOM)(PMID:21642991) and tumor predisposition syndrome (TPDS)(PMID:21874003). The all BAP1s except those of C. quinquefasciatus have a molecular mass of more than 90 kDa(PMID:22374320).

Publications

Species	Application	Title
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB	iScience; Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4; Authors - Chenggang Zhang
mouse	WB	Nature; Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase; Authors - Weiran Ge