Anticorps Polyclonal de lapin anti-BAP29

BAP29 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15796-1-PBS

Synonymes

BCR-associated protein 29, B-cell receptor-associated protein 29, BCAP29

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Galerie de données de validation

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  WB analysis using 15796-1-AP (same clone as 15796-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 15796-1-AP (same clone as 15796-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 15796-1-AP (same clone as 15796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 15796-1-AP (same clone as 15796-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15796-1-AP (same clone as 15796-1-PBS)

  Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 15796-1-AP (same clone as 15796-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug BAP29 Polyclonal antibody (15796-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15796-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15796-1-PBS cible BAP29 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAP29 Protéine recombinante Ag8505
• Nom complet: B-cell receptor-associated protein 29
• Masse moléculaire calculée: 241 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC008478
• Symbole du gène: BAP29
• Identification du gène (NCBI): 55973
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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