Anticorps Polyclonal de lapin anti-BARD1

BARD1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 22964-1-AP

Synonymes

BARD 1, BARD-1, BRCA1 associated RING domain 1, BRCA1-associated RING domain protein 1, EC:2.3.2.27

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 22964-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22964-1-AP (BARD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

22964-1-AP cible BARD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BARD1 Protéine recombinante Ag18965
• Nom complet: BRCA1 associated RING domain 1
• Masse moléculaire calculée: 777 aa, 87 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC126426
• Symbole du gène: BARD1
• Identification du gène (NCBI): 580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BARD1 (BRCA1-associated RING domain protein 1) is a multifunctional protein that interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. Moreover, BARD1 is central to DNA repair, tumor suppression, and cancer predisposition.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu
human	WB	Molecules; FOXO3a Mediates Homologous Recombination Repair (HRR) via Transcriptional Activation of MRE11, BRCA1, BRIP1, and RAD50; Authors - Gozde Inci
human	WB	Am J Physiol Cell Physiol; Synergistic Effects of Olaparib and Palbociclib in Resistant epithelial ovarian cancer; Authors - Shuo Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Vikas (Verified Customer) (04-08-2025)

worked very well. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: HEK-293

{{ptg:RelatedPrimaryAntibodies}}