Anticorps Recombinant de lapin anti-BARHL2

BARHL2 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240558G8

N° de cat : 83596-4-PBS

Synonymes

240558G8, BarH like 2 homeobox protein, BarH-like 2 homeobox protein

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Galerie de données de validation

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  WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00582-2

  Cytometric bead array standard curve of MP00582-2, BARHL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83596-1-PBS. Detection antibody: 83596-4-PBS. Standard: Ag21156. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83596-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

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Informations sur le produit

83596-4-PBS cible BARHL2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: BARHL2 Protéine recombinante Ag21156
• Nom complet: BarH-like homeobox 2
• Masse moléculaire calculée: 387 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC126439
• Symbole du gène: BARHL2
• Identification du gène (NCBI): 343472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

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