Anticorps Polyclonal de lapin anti-BAX

BAX Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(50)

N° de cat : 23931-1-AP

Synonymes

Apoptosis regulator BAX, BAX

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Galerie de données de validation

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  WB analysis of MCF-7 using 23931-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 23931-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW264.7 using 23931-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 23931-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23931-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23931-1-AP (BAX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 23931-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23931-1-AP (BAX Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 23931-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23931-1-AP (BAX Antibody) at dilution of 1:50 (under 40x lens).

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Applications publiées

• WB: See 45 publications below
• IHC: See 2 publications below

Informations sur le produit

23931-1-AP cible BAX dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAX Protéine recombinante Ag21068
• Nom complet: BCL2-associated X protein
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: BC014175
• Symbole du gène: BAX
• Identification du gène (NCBI): 581
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).

Publications

Species	Application	Title
mouse	WB	Br J Pharmacol; Astaxanthin attenuates hepatic damage and mitochondrial dysfunction in non-alcoholic fatty liver disease by up-regulating the FGF21/PGC-1α pathway.; Authors - Liwei Wu
human	WB	Oncotarget; By reducing hexokinase 2, resveratrol induces apoptosis in HCC cells addicted to aerobic glycolysis and inhibits tumor growth in mice.; Authors - Weiqi Dai
human	WB	Aging (Albany NY); SRT2183 impairs ovarian cancer by facilitating autophagy.; Authors - Tingting Sun
human	WB	Int J Oncol; NT21MP negatively regulates paclitaxel-resistant cells by targeting miR‑155‑3p and miR‑155-5p via the CXCR4 pathway in breast cancer.; Authors - Yueyue Wang
human	WB	Nanoscale Res Lett; Cytotoxicity induced by nanobacteria and nanohydroxyapatites in human choriocarcinoma cells.; Authors - Mingjun Zhang
human	WB	Oncotarget; AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells.; Authors - Yarong Song

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