- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-BAX
BAX Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (6)
Applications
WB, IP, IHC, FC, CoIP, chIP, ELISA
Conjugaison
Non conjugué
2848
N° de cat : 50599-2-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293, cellules COLO 320, cellules HeLa, cellules HT-1080, cellules NIH/3T3, cellules PC-12, cellules Raji, cellules RAW 264.7, tissu cardiaque de souris, tissu cérébral de rat, tissu de côlon de rat, tissu pulmonaire de rat, tissu rénal de rat, tissu rénal de souris |
| Résultats positifs en IP | cellules Raji, tissu cardiaque de souris |
| Résultats positifs en IHC | tissu pulmonaire de souris, tissu rénal de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en cytométrie | cellules Ramos, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
50599-2-Ig cible BAX dans les applications de WB, IP, IHC, FC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Chèvre, Humain, Lapin, poisson-zèbre, porc, poulet, souris, Hamster |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | BAX Protéine recombinante Ag0576 |
| Nom complet | BCL2-associated X protein |
| Masse moléculaire calculée | 21 kDa |
| Poids moléculaire observé | 21 kDa |
| Numéro d’acquisition GenBank | BC014175 |
| Symbole du gène | BAX |
| Identification du gène (NCBI) | 581 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL (PMIDs: 28755482, 21458670). In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion (PMID:10766872). The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C (PMID:25458844).
BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites (PMID:7834749). Furthermore, p53 directly interacts with BAX to promote its activation (PMID:14963330).
What is the molecular weight of BAX?
BAX is a 192 amino acid protein that has a predicted molecular weight of 21.1 kDa.
What is the subcellular localization of BAX?
In healthy mammalian cells, BAX is localized to the cytoplasm. In response to apoptotic stimuli, BAX translocates to the outer mitochondrial membrane.
What is the tissue specificity of BAX?
BAX is ubiquitously expressed (PMID: 25613900).
What is the connection between BAX and cancer?
BAX appears to play an important role in suppressing cancer development, and decreased BAX levels are associated with chemo- and radioresistance in a number of cancers including lung cancer, chronic lymphocytic leukemia (CLL), and prostate cancer. Loss-of-function mutations of BAX have also been reported in hematopoietic malignancies in humans (PMID:9531611) and in gastrointestinal cancer of the microsatellite mutator phenotype (MMP), where BAX inactivation contributes to tumor progression by providing a survival advantage (PMID:9331106).
A number of anticancer drugs used clinically have been demonstrated to induce BAX activation indirectly to facilitate apoptosis of tumor cells. Recent efforts have demonstrated that BAX itself may be a promising direct target for small-molecule drug discovery for novel anticancer drugs and several direct BAX activators have been identified that may hold promise for cancer therapy, with the potential to overcome chemo- and radioresistance (PMID:25230299).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for BAX antibody 50599-2-Ig | Download protocol |
| IHC protocol for BAX antibody 50599-2-Ig | Download protocol |
| IP protocol for BAX antibody 50599-2-Ig | Download protocol |
| FC protocol for BAX antibody 50599-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma | ||
ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Cell Res Tom20 senses iron-activated ROS signaling to promote melanoma cell pyroptosis.
| ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Macarena Lucia (Verified Customer) (10-17-2022) | nice band
|
FH ZEE (Verified Customer) (07-13-2020) | VERY GOOD antibody when western
|
FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
|
FH Kathryn (Verified Customer) (11-04-2019) | Image shows a post-natal day 2 mouse ovary with germ cells marked by TRA98 (Abcam) in green, and BAX (Proteintech) marked in red.
 |
